Differential impedance spectrometer and vision system for analysis of single cells

This paper reports on a differential impedance spectroscopy system for single-cell analysis, which also features an optical setup with machine-vision that simultaneously provides optical data for a direct comparison. Such a combined setup allows for benchmarking the differential impedance spectroscopy against the optical data in a quantitative way. Here we present simultaneously acquired optical and impedance-spectroscopic data of yeast cells and polystyrene beads. Signal simulations have been performed and fitted to the measured data to increase the quality of the results and to explain the recorded signal shapes.