Vacuum supported liquid waste handling for dna extraction on centrifugally operated Lab-on-a-Chip systems

Author

Mark, D. ; Haeberle, S. ; Lutz, S. ; Zengerle, R. ; von Stetten, F.

Author_Institution

HSG-IMIT-Inst. for Micromachining & Inf. Technol., Villingen-Schwenningen, Germany

fYear

2009

fDate

21-25 June 2009

Firstpage

1230

Lastpage

1233

Abstract

We present a reliable liquid waste containment for centrifugally operated lab-on-a-chip systems that works even for highly wetting reagents. It is based on the passive generation and enclosure of vacuum in a closed storage chamber that prevents all liquids from capillary reflux into the microfluidic channel network. The new waste handling presented here enabled the implementation of an integrated deoxyribonucleic acid (DNA) extraction chemistry without contamination risks based on purely passive structures. Using a 32 muL whole blood sample we achieved an extraction of 290 ng plusmn 80 ng DNA in 100 muL of eluate.

Keywords

DNA; capillarity; lab-on-a-chip; microfluidics; wastewater treatment; DNA extraction; capillary reflux; deoxyribonucleic acid; lab-on-a-chip; liquid waste containment; microfluidic channel; waste handling; Chemistry; Contamination; DNA; Lab-on-a-chip; Laboratories; Liquids; Microfluidics; Purification; Vacuum systems; Waste handling; DNA-extraction; lab-on-a-chip; microfluidics; self-sealing; waste handling;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International

Conference_Location

Denver, CO

Print_ISBN

978-1-4244-4190-7

Electronic_ISBN

978-1-4244-4193-8

Type

conf

DOI

10.1109/SENSOR.2009.5285880

Filename

5285880