Improvement of detection sensitivity using electrodes with micropillar structures

Author

Ginoza, T. ; Okamura, K. ; Fukuda, J. ; Suzuki, H.

Author_Institution

Grad. Sch. of Pure & Appl. Sci., Univ. of Tsukuba, Tsukuba, Japan

fYear

2009

fDate

21-25 June 2009

Firstpage

1253

Lastpage

1256

Abstract

Electrochemical biosensing has been generally considered to be highly efficient. However three-dimensional numerical analysis has revealed that only a fraction of an analyte is consumed for amperometric detection in a flowing stream. To improve the collection efficiency and the sensitivity of detection, micropillar electrodes were fabricated. Furthermore, a gold black layer was formed on the surface of the micropillars. In the best case, a 22-fold increase was observed in the generated current compared with a flat electrode. The micropillar electrodes were also used to conduct an electrochemical enzyme linked-immunosorbent assay.

Keywords

amperometric sensors; bioMEMS; biosensors; computational fluid dynamics; electrochemical electrodes; enzymes; gold; 3D numerical analysis; amperometric detection; electrochemical biosensing; electrochemical enzyme linked-immunosorbent assay; electrodes detection sensitivity; micropillar electrodes; micropillar structures; Biosensors; Electrodes; Electrons; Glass; Gold; Immune system; Insulation; Sputtering; Substrates; Testing; Amperometry; Bone metabolic marker; Enzyme-linked immunosorbent assay; Flow channel; Gold black; Micropillars;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International

Conference_Location

Denver, CO

Print_ISBN

978-1-4244-4190-7

Electronic_ISBN

978-1-4244-4193-8

Type

conf

DOI

10.1109/SENSOR.2009.5285886

Filename

5285886