Title :
Measurements of argon laser light attenuation in the skin `in vivo´ using a unique animal model
Author :
Ghaffari, Shirin ; Welch, A.J. ; Diller, K.R.
Abstract :
A dorsal skin flap chamber model for microcirculation studies is discussed. It permits direct irradiation of subcutaneous arterioles and venules in vivo as well as irradiation of the same vessels through the skin of unanesthetized hamsters. A system has been developed to permit vital and video microscopy while the microvascular system is being irradiated with laser light. Selected venules have been irradiated with an argon laser adjusted to deliver 1 to 55 J/cm2 on a 250-μm-diameter spot during a 20-ms irradiation. Video microscopy evaluates the creation of embolized or stationary coaguli. To produce these coaguli by irradiating through the skin (average thickness 0.4 mm) requires 1.5 times the energy density required when the venules are being irradiated directly. The different energy densities required to produce similar damage indicate the role of light attenuation in the skin
Keywords :
biophysics; light absorption; skin; 0.4 mm; 250 micron; Ar laser light attenuation; animal model; coaguli; dorsal skin flap chamber model; energy density; microcirculation studies; subcutaneous arterioles; unanesthetized hamsters; venules; video microscopy; vital microscopy; Animals; Argon; Attenuation measurement; Biomedical measurements; In vivo; Laser modes; Microscopy; Optical attenuators; Skin; Windows;
Conference_Titel :
Engineering in Medicine and Biology Society, 1989. Images of the Twenty-First Century., Proceedings of the Annual International Conference of the IEEE Engineering in
Conference_Location :
Seattle, WA
DOI :
10.1109/IEMBS.1989.96436
