Title :
Microsystems for determining the heterogeniety of head and neck cancer cell populations by para-magnetophoresis
Author :
Jung, J. ; Wang, D. ; Chen, G. ; Shin, D. ; Han, K.H. ; Frazier, A.B.
Author_Institution :
Georgia Inst. of Technol., Atlanta, GA, USA
Abstract :
This paper is focused on the design, fabrication and characterization of a continuous flow magnetophoretic microseparator for the separation of selectively tagged cells using para-magnetophoresis. The microseparator was characterized experimentally using head and neck cells lines 212LN and 686LN-M4E as a case study. The microfluidic separation system can separate the selectively tagged cancer cells depending on their membrane protein expression level. Experimental results show that at flow rates of 100 muL/hr and 200 muL/hr, 86.3% and 79.0% of tagged cancer cells were attracted toward center channel respectively, while 95.1% and 87.2% of non-tagged cells remained in the side channels. Additionally, the experimental results of separating tagged cancerous cells from human blood were presented.
Keywords :
bioMEMS; biomagnetism; biomembranes; blood; cancer; cellular biophysics; microchannel flow; microfabrication; paramagnetism; proteins; tumours; continuous flow magnetophoretic microseparator fabrication; head-and-neck cancer cell populations; human blood; membrane protein expression level; microchannel flow; microfluidic separation system; paramagnetophoresis; Biomembranes; Cancer; Cells (biology); Fabrication; Humans; Magnetic heads; Magnetic separation; Microfluidics; Neck; Proteins; Blood; Cancer Cell Separator; Magnetophoresis; Microfluidic System;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International
Conference_Location :
Denver, CO
Print_ISBN :
978-1-4244-4190-7
Electronic_ISBN :
978-1-4244-4193-8
DOI :
10.1109/SENSOR.2009.5285968