Title :
TISSUE-MIMICKING IN VITRO ANALYSIS SYSTEM to exclusively stimulate single cell and detect its physiological reaction
Author :
Okonogi, Atsuhito ; Terao, Kyohei ; Okitsu, Teru ; Suzuki, Takaaki ; Kotera, Hidetoshi
Author_Institution :
JST Crest, Japan
Abstract :
In this paper, we present imaging of the physiological cell reaction of stimulus-responsive cells using a new orifice channel chip named the TISSUE-MIMICKING IN VITRO ANALYSIS SYSTEM. The chip enables designated cells treatment using orifice-channels similar to blood vessels, and cell-array like a tissue: cells are along on the blood vessels. These cell handling is an important part in the observation of regulation of cell-functions on stimulus-responsive cells (e.g. pancreas). To study this role, we address to detect cellular stimulation response to designated stimulation using the chip.
Keywords :
bioMEMS; biological techniques; biomimetics; cellular biophysics; lab-on-a-chip; Tissue Mimicking In Vitro Analysis System; blood vessel mimic; cell array mimic; cell functions regulation; orifice channel chip; single cell physiological reaction; single cell stimulation; stimulus responsive cells; Biomedical imaging; Blood vessels; Cells (biology); Image analysis; In vitro; Orifices; Pancreas; Plasma applications; Plasma devices; Sugar; cell array device; cellular stimulation-response phenomena; fluorescently Visualizing of cell-response; imaging; single cell stimulation;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International
Conference_Location :
Denver, CO
Print_ISBN :
978-1-4244-4190-7
Electronic_ISBN :
978-1-4244-4193-8
DOI :
10.1109/SENSOR.2009.5285969
