High-throughput fully automated microrobotic zebrafish embryo injection

Author

Wang, W.H. ; Liu, X.Y. ; Sun, Y.

Author_Institution

Mech. Eng., Univ. of Canterbury, Christchurch

fYear

2008

fDate

19-23 May 2008

Firstpage

1768

Lastpage

1769

Abstract

Injection of foreign materials (e.g., DNA, RNAi, sperm, protein, and drug compounds) into individual cells has significant implications in genetics, transgenics, assisted reproduction, and drug discovery. This video presents a microrobotic system for fully automated zebraflsh embryo injection, which overcomes the problems inherent in manual operation, such as human fatigue and large variations in success rates due to poor reproducibility. Based on computer vision and motion control, the automated microrobotic system is capable of immobilizing a large number of zebraflsh embryos into a regular pattern within seconds and injecting 15 embryos (chorion unremoved) per minute with a success rate, survival rate, and phenotypic rate all close to 100%.

Keywords

medical robotics; microrobots; motion control; robot vision; computer vision; foreign materials; fully automated microrobotic zebrafish embryo injection; human fatigue; manual operation; motion control; Computer vision; DNA; Drugs; Embryo; Fatigue; Genetics; Humans; Manuals; Proteins; Reproducibility of results; Microrobotic control; cell injection; computer vision; high throughput; no-tail phenotype; zebrafish embryos;

fLanguage

English

Publisher

ieee

Conference_Titel

Robotics and Automation, 2008. ICRA 2008. IEEE International Conference on

Conference_Location

Pasadena, CA

ISSN

1050-4729

Print_ISBN

978-1-4244-1646-2

Electronic_ISBN

1050-4729

Type

conf

DOI

10.1109/ROBOT.2008.4543457

Filename

4543457