Title :
Ultrasensitive detection of specific sequences in double-stranded DNA by laser-induced fluorescence
Author :
Castro, A. ; Paz-Matos, L.
Author_Institution :
Los Alamos Nat. Lab., NM, USA
Abstract :
Summary form only given. Here, we describe a new method for the direct detection of specific sequences in double-stranded DNA. This method allows analysis of genomic samples without the need for a denaturing step. In addition, it can be combined with other single-molecule detection techniques that allow sizing DNA fragments by measuring their individual electrophoretic velocities. The present technique is based on a two-color, single fluorescent molecule detection technique.
Keywords :
DNA; biomolecular effects of radiation; fluorescence; genetics; biological sample; biomedical diagnosis; double-stranded DNA; electrophoretic velocity; fragment sizing; gene detection; laser-induced fluorescence; sequence detection; single molecule detection; Aerosols; Atmospheric measurements; DNA; Diseases; Fluorescence; Laboratories; Microorganisms; Particle measurements; Probes; Sequences;
Conference_Titel :
Lasers and Electro-Optics, 1999. CLEO '99. Summaries of Papers Presented at the Conference on
Conference_Location :
Baltimore, MD, USA
Print_ISBN :
1-55752-595-1
DOI :
10.1109/CLEO.1999.834152
