Title :
A microfabricated, flow driven mill for the mechanical lysis of algae
Author :
Millis, Justin ; Connell, Laurie ; Collins, Scott D. ; Smith, Rosemary L.
Author_Institution :
MicroInstruments & Syst. Lab., Univ. of Maine, Orono, ME, USA
Abstract :
This paper reports a novel, new means of mechanically lysing algae using a microfabricated, flow-driven grinding mill. The mill is demonstrated to be effective in the mechanical lysis of the dinoflagellate genus Alexandrium, a neurotoxin producing algae, responsible for Red Tide and paralytic shellfish poisoning. Pre and post milling cell counts revealed lysing efficiencies as high as 96%. Selective amplification of lysate samples was successfully performed, using Polymerase Chain Reaction (PCR) and primers specific to Alexandrium, demonstrating that the released DNA is suitable for downstream analysis.
Keywords :
bioMEMS; biochemistry; biological techniques; microfabrication; microorganisms; toxicology; DNA; PCR; Polymerase Chain Reaction; Red Tide; dinoflagellate genus Alexandrium; downstream analysis; flow-driven grinding mill; lysate samples; lysing efficiencies; mechanically lysing algae; microfabricated mill; neurotoxin; paralytic shellfish poisoning; post milling cell counts; pre milling cell counts; selective amplification; Algae; Blades; DNA; Fluorescence; Glass; Silicon; Surface treatment;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2015 28th IEEE International Conference on
Conference_Location :
Estoril
DOI :
10.1109/MEMSYS.2015.7050918