Droplet flow focusing for molecular binding detection

Author

Sunggu Kim ; Junghoon Lee

Author_Institution

Seoul Nat. Univ., Seoul, South Korea

fYear

2015

fDate

18-22 Jan. 2015

Firstpage

230

Lastpage

233

Abstract

We report the detection of interfacial molecular binding via droplet generation in a flow focusing device. DNA hybridization at the phospholipid causes interfacial tension shift at the oil-water boundary which changes the mode of droplet production in the device. Droplet size decreased ~30% due to a complementary binding compared with non-complementary and control cases. Our report includes a molecular protocol to functionalize the interface with single-strand (ss) DNA as receptor and flow condition tuning for an unambiguous distinction of complementary binding.

Keywords

DNA; bioMEMS; biosensors; drops; flow sensors; lipid bilayers; microfluidics; microsensors; molecular biophysics; molecular configurations; surface tension; DNA hybridization; complementary binding; droplet flow focusing device; droplet generation; droplet production mode; droplet size; flow condition; interfacial molecular binding detection; interfacial tension shift; molecular protocol; oil-water boundary; phospholipid; receptor; single-strand DNA; unambiguous distinction; DNA; Fluorescence; Focusing; Lipidomics; Microchannels; Microfluidics; Protocols;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2015 28th IEEE International Conference on

Conference_Location

Estoril

Type

conf

DOI

10.1109/MEMSYS.2015.7050929

Filename

7050929