Skin-equivalent integrated with perfusable channels on curved surface

This conference proceeding describes a method to construct a skin-equivalent cultured on a curved surface. The skin-equivalent consists of not only the dermis/epidermis but also perfusable channels. We embedded an anchoring structure in a culture device to prevent the horizontal contraction of the tissue during cultivation. Owing to perfusion of culture medium via the channels, we can culture the epidermis at the air-liquid interface for cornification. This method enables the skin-equivalent construction on a curved surface; the curved surface is necessary for the construction of 3D complex skin surface and the covering of living skin on the 3D surface of biohybrid robots.