In vitro dynamic fertilization by using EWOD device

Author

Lung-Yuan Chung ; Hsien-Hua Shen ; Yu-Hsiang Chung ; Chih-Chen Chen ; Chia-Hsien Hsu ; Hong-Yuan Huang ; Da-Jeng Yao

Author_Institution

Inst. of NanoEngineering & Microsyst., Nat. Tsing Hua Univ., Hsinchu, Taiwan

fYear

2015

fDate

18-22 Jan. 2015

Firstpage

519

Lastpage

522

Abstract

This research demonstrates the in vitro fertilization (IVF) technique by using the digital microfluidic (DMF) system. The DMF device has been proved with biocompatibility and used in this research for mouse gametes in vitro fertilization and embryos culture based on the dispersed droplet form. Dynamically moving, cell culture and observation can be achieved by the time-lapse microscopy in an incubator. The fertilization rate of IVF on DMF is 34.8%, and about 25% inseminated embryos dynamically cultured on DMF chip could develop into 8-cell stage. The result indicated that the DMF system has the potential used for assisted reproductive technology.

Keywords

bioMEMS; cell motility; drops; electrochemistry; microfluidics; optical microscopy; wetting; 8-cell stage; DMF chip; EWOD device; assisted reproductive technology; biocompatibility; digital microfluidic system; dispersed droplet form; dynamically moving cell culture; embryos culture; in vitro dynamic fertilization; incubator; inseminated embryos; mouse gametes in vitro fertilization; time-lapse microscopy; Dielectrics; Electrodes; Embryo; In vitro fertilization; Mice; Microfluidics; Microscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2015 28th IEEE International Conference on

Conference_Location

Estoril

Type

conf

DOI

10.1109/MEMSYS.2015.7051006

Filename

7051006