Title :
In vitro dynamic fertilization by using EWOD device
Author :
Lung-Yuan Chung ; Hsien-Hua Shen ; Yu-Hsiang Chung ; Chih-Chen Chen ; Chia-Hsien Hsu ; Hong-Yuan Huang ; Da-Jeng Yao
Author_Institution :
Inst. of NanoEngineering & Microsyst., Nat. Tsing Hua Univ., Hsinchu, Taiwan
Abstract :
This research demonstrates the in vitro fertilization (IVF) technique by using the digital microfluidic (DMF) system. The DMF device has been proved with biocompatibility and used in this research for mouse gametes in vitro fertilization and embryos culture based on the dispersed droplet form. Dynamically moving, cell culture and observation can be achieved by the time-lapse microscopy in an incubator. The fertilization rate of IVF on DMF is 34.8%, and about 25% inseminated embryos dynamically cultured on DMF chip could develop into 8-cell stage. The result indicated that the DMF system has the potential used for assisted reproductive technology.
Keywords :
bioMEMS; cell motility; drops; electrochemistry; microfluidics; optical microscopy; wetting; 8-cell stage; DMF chip; EWOD device; assisted reproductive technology; biocompatibility; digital microfluidic system; dispersed droplet form; dynamically moving cell culture; embryos culture; in vitro dynamic fertilization; incubator; inseminated embryos; mouse gametes in vitro fertilization; time-lapse microscopy; Dielectrics; Electrodes; Embryo; In vitro fertilization; Mice; Microfluidics; Microscopy;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2015 28th IEEE International Conference on
Conference_Location :
Estoril
DOI :
10.1109/MEMSYS.2015.7051006
