Title :
Serial Algorithm for high-speed autofocusing of cells using Depth From Diffraction (DFDi) method
Author :
Makise, Soshiro ; Oku, Hiromasa ; Ishikawa, Masatoshi
Author_Institution :
Dept. of Inf. Phys. & Comput., Univ. of Tokyo, Tokyo
Abstract :
In this paper, a new serial algorithm for highspeed autofocusing of cells is proposed. The proposed algorithm extracts depth information of target cells from their diffraction patterns. High-speed focusing of yeast cells with 20 ms response time was demonstrated. Continuous autofocusing of yeast cells with a scanning microscope was also demonstrated. The successful continuous autofocusing suggests that image-based high- throughput measurement of cells could be realized using the proposed algorithm.
Keywords :
cellular biophysics; medical control systems; optical focusing; optical microscopes; depth from diffraction; high-speed cells autofocusing; image-based high-throughput measurement; scanning microscope; serial algorithm; yeast cells; Diffraction;
Conference_Titel :
Robotics and Automation, 2008. ICRA 2008. IEEE International Conference on
Conference_Location :
Pasadena, CA
Print_ISBN :
978-1-4244-1646-2
Electronic_ISBN :
1050-4729
DOI :
10.1109/ROBOT.2008.4543686
