Title :
Sensitive detection of 2,4,6-trinitrotoluene by surface plasmon fluorescence spectroscopy
Author :
Ito, Satoshi ; Tanaka, Shuhei ; Yatabe, Rui ; Onodera, Takeshi ; Toko, Kiyoshi
Author_Institution :
Grad. Sch. of Inf. Sci. & Electr. Eng., Kyushu Univ., Fukuoka, Japan
Abstract :
We developed a compact measurement system for surface plasmon fluorescence spectroscopy (SPFS) and a sensor surface for the detection of 2,4,6-trinitrotoluene (TNT). We employed a laser diode as a light source in the optical system for exciting fluorescence. Then, a fan-shaped beam was irradiated from the laser diode to a Au thin film using a beam expander. Fluorescence emission was captured using a photomultiplier detector. The sensor surface was modified with TNT analogues via a self-assembled monolayer. A limit of detection in the ppt range for TNT was obtained using a fluorescence-labeled anti-TNT monoclonal antibody in the SPFS system by indirect competitive assay.
Keywords :
chemical sensors; fluorescence; fluorescence spectroscopy; gold; light sources; metallic thin films; molecular biophysics; monolayers; photomultipliers; proteins; self-assembly; semiconductor lasers; spectrochemical analysis; surface plasmons; thin film sensors; 2,4,6-trinitrotoluene detection; Au; Au thin film; TNT analogues; beam expander; compact measurement system; detection limit; fan-shaped beam; fluorescence emission; fluorescence-labeled anti-TNT monoclonal antibody; indirect competitive assay; laser diode; light source; optical system; photomultiplier detector; self-assembled monolayer; sensor surface; surface plasmon fluorescence spectroscopy; Diode lasers; Fluorescence; Gold; Laser beams; Lenses; Measurement by laser beam; Plasmons; fluorescence; surface plasmon resonance; surface plasmon-field enhanced fluorescence spectroscopy; trinitrotoluene;
Conference_Titel :
SENSORS, 2014 IEEE
Conference_Location :
Valencia
DOI :
10.1109/ICSENS.2014.6984999
