Title :
Potentiometric detection of protein interactions with peptide aptamers
Author :
Estrela, Pedro ; Paul, Debjani ; Migliorato, Piero ; Ferrigno, Paul Ko ; Wang, Ling ; Huq, Ejaz
Author_Institution :
Dept. of Eng., Univ. of Cambridge, Cambridge
Abstract :
Label-free electrical detection of protein interactions has been achieved by direct measurement of variations in open circuit potential using an accurate differential voltage measurement as well as field-effect transistors. Our model system involves a panel of peptide aptamers that recognise specific protein partners of the cyclin-dependent kinase family, which are relevant to cancer research applications.
Keywords :
bioelectric phenomena; biosensors; field effect transistors; molecular biophysics; potentiometers; proteins; Label-free electrical detection; differential voltage measurement; field-effect transistors; open circuit potential; peptide aptamers; potentiometric detection; protein interactions; Cancer; Charge measurement; Circuits; Current measurement; Electrical resistance measurement; FETs; Instruments; Peptides; Protein engineering; Voltage measurement;
Conference_Titel :
Sensors, 2008 IEEE
Conference_Location :
Lecce
Print_ISBN :
978-1-4244-2580-8
Electronic_ISBN :
1930-0395
DOI :
10.1109/ICSENS.2008.4716524
