Title :
Convection-based realtime polymerase chain reaction (PCR) utilizing transparent graphene heaters
Author :
Kwang Hyo Chung ; Yo Han Choi ; Hong Kyw Choi ; Jin Tae Kim ; Young-Jun Yu ; Jin Sik Choi ; Doo-Hyeb Youn ; Choon-Gi Choi
Author_Institution :
Graphene Electron. Creative Res. Center, South Korea
Abstract :
We present a polymerase chain reaction (PCR) chip incorporating graphene heaters and thermal convection. We adopted graphene heaters in order to replace the bulky metal heating blocks or opaque metal line heaters utilized in the previous PCR chips. Transparency of the CVD based graphene heaters allowed the realtime fluorescence detection of PCR product using a light-path passing through a reaction chamber. Thermal convection enabled rapid and automated PCR thermocycling.
Keywords :
chemical vapour deposition; convection; fluorescence; graphene; optical sensors; temperature sensors; C; CVD; PCR chip; automated PCR thermocycling; bulky metal heating block; convection-based realtime polymerase chain reaction; opaque metal line heater; reaction chamber; realtime fluorescence detection; thermal convection; transparent graphene heater; DNA; Fluorescence; Glass; Graphene; Heating; Metals; Polymers; PCR; convection; graphene; heater;
Conference_Titel :
SENSORS, 2014 IEEE
Conference_Location :
Valencia
DOI :
10.1109/ICSENS.2014.6985173
