A non-invasive flexible multi-channel electrode for in vivo mouse EEG recording

Although stainless steel screw is widely used in various mouse electroencephalogram (EEG) researches, it is potentially dangerous to be implanted in young mice or disease models due to the high risks of bleeding, infection, or traumatic injury. In this paper, we introduce a non-invasive flexible multi-channel electrode for sensory evoked potential recording to alleviate the risk of screw implantation in mice. The electrode has been designed to stably sit on the scalp and cover the curvature of scalp without the need of screws. In addition, the electrode guarantees reusability such that it can be attached and detached conveniently for usage on the scalp of multiple mice. We have designed a sixteen-channel electrode array to analyze sensory evoked potential recording spatiotemporally. We further verify its performance by conducting an experiment related to the resting state, attaching the needle on the right hindlimb, and electrical stimulation of the right hindlimb.