Plug dispersion compensation for moving field capillary electrophoresis by low voltage electroosmotic pump

In this work, we present a method to compensate the plug dispersion for the moving field capillary electrophoresis (CE) application. A forward electroosmotic pump (EOP) will be employed to compensate the back flow in the active separation segment. The required driving voltage of the EOP was analyzed as a function of the channel topology. Two channel topologies are proposed and investigated with the aim of reducing the EOP voltage level. The finite element modeling was performed and the numerical simulation results are compared with the analytical calculation results. The preliminary experimental results were retrieved by the PIV technique.