Removal of nonspecific bindings in on-chip ELISAs with low power ultrasound

Author

Brandhoff, Lukas ; Vellekoop, Michael J. ; Redl, Heinz ; Haller, Anna ; Zirath, Helene ; Peham, Johannes ; Wiesinger-Mayr, Herbert ; Spittler, Andreas ; Schnetz, Guntram

Author_Institution

Inst. for Microsensors, -Actuators, & -Syst., (IMSAS/MCB), Univ. of Bremen, Bremen, Germany

fYear

2014

fDate

2-5 Nov. 2014

Firstpage

1862

Lastpage

1865

Abstract

We present a novel method for removing nonspecifically bound proteins for on-chip ELISAs (Enzyme-Linked-Immuno-Sorbent-Assays) by integrated ultrasonic washing of the reaction chamber. Low power ultrasound is concentrated into the reaction chamber with an ultrasonic horn contained in the base of the measurement system. Application of ultrasonication during the last washing step of the ELISA protocol removes adsorbed proteins from the wall while not affecting specifically bound analytes. This method reduces the cost of the disposable assay-chips, while delivering comparable results with standard blocking methods.

Keywords

adsorption; biochemistry; biological techniques; biosensors; bonds (chemical); desorption; enzymes; lab-on-a-chip; molecular biophysics; spectrochemical analysis; surface cleaning; ultrasonic applications; ELISA protocol; disposable assay-chip cost reduction; enzyme-linked-immuno-sorbent-assay; integrated ultrasonic washing; low power ultrasound concentration; measurement system; nonspecific protein binding removal; on-chip ELISA nonspecific binding removal; protein adsorption; reaction chamber; specific analyte binding; standard blocking method; ultrasonic horn; ultrasonication application; Acoustics; Fluorescence; Microfluidics; Proteins; System-on-chip; Ultrasonic imaging; Ultrasonic variables measurement; ELISA; Immunoassay; Lab-On-a-Chip; Sonication; Ultrasonic Horn; Ultrasound;

fLanguage

English

Publisher

ieee

Conference_Titel

SENSORS, 2014 IEEE

Conference_Location

Valencia

Type

conf

DOI

10.1109/ICSENS.2014.6985391

Filename

6985391