Resolution of amino acid enantiomers by chiral solvent efluents on the thin layer chromatography

Enantiomeric resolution of aromatic and aliphatic amino acid into L-amino acid and D-amino acid via TLC is achieved by using chiral mobile phase as thin layer chromatographic eluent. For this purpose, Ethyl(S)-(+)-lactate as a chiral developing solvent was chosen to afford effective separation on the TLC plates. The peculiarities of the chromatographic behavior of ethylacetate-amino acid interaction and the mechanism of their retention in TLC are discussed. Methods were developed for the determination of amino acid enantiomers based on the discrepancies of three-point interaction for chiral solvent between D- and L-amino acid enantiomers respectively. The effects of mobile phase composition and temperature on the retention are also examined. The procedure are characterized by simplicity, efficiency and relatively low cost to analyze enantiomers of some amino acids.