Title :
Role of Vector in The Dynamics of Antisense Activity
Author :
Sundaram, Sumati ; Lee, Li Kim ; Roth, Charles M.
Author_Institution :
Department of Chemical and Biochemical Engineering, Rutgers University, piscataway, NJ, USA
Abstract :
The objective of this work is to investigate the role of the vector in the dynamics of antisense effects. Polymer/DNA complexs between cationic, branched polymer, polyethylenimine (PEI) at a range of molecular weights and oligonucleotides (ODNs), with varying backbone chemistries, were studied using a variety of physico-chemical and cellular assays. The extent and time scale of antisense effects were found to depend strongly on both the polymer molecular weight as well as the ODN chemistry. The optimum molecular weight of PEI for maximum ODN delivery depends strongly on the ODN chemistry.
Keywords :
GFP down-regulation; antisense; cellular uptake; polyethlenimine; Biomedical engineering; Chemistry; DNA; Fluorescence; Kinetic theory; Lipidomics; Polymers; Production; Proteins; Sequences; GFP down-regulation; antisense; cellular uptake; polyethlenimine;
Conference_Titel :
Bioengineering Conference, 2006. Proceedings of the IEEE 32nd Annual Northeast
Conference_Location :
Easton, PA, USA
Print_ISBN :
0-7803-9563-8
DOI :
10.1109/NEBC.2006.1629812
