Quantifying molecular colocalization in live cell fluorescence microscopy

Author

Humpert, Fabian ; Yahiatene, Idir ; Lummer, Martina ; Sauer, Matthias ; Huser, T.

Author_Institution

Biomol. Photonics, Bielefeld Univ., Bielefeld, Germany

fYear

2013

fDate

12-16 May 2013

Firstpage

1

Lastpage

1

Abstract

One of the most challenging tasks in microscopy is the quantitative identification and characterization of molecular interactions. In living cells this task is typically performed by fluorescent labeling of the interaction partners with spectrally distinct fluorophores and imaging in different color channels. Potential interaction can then be determined by parameters that can measure the degree of molecular colocalization.

Keywords

cellular biophysics; fluorescence; molecular biophysics; molecular configurations; optical microscopy; proteins; color channel imaging; fluorescent labeling; live cell fluorescence microscopy; molecular colocalization; molecular interactions; potential interaction; quantitative characterization; quantitative identification; spectrally distinct fluorophores; Colored noise; Correlation; Educational institutions; Image color analysis; Microscopy; Noise level; Signal to noise ratio;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics Europe (CLEO EUROPE/IQEC), 2013 Conference on and International Quantum Electronics Conference

Conference_Location

Munich

Print_ISBN

978-1-4799-0593-5

Type

conf

DOI

10.1109/CLEOE-IQEC.2013.6801498

Filename

6801498