Title :
Precise quantification and control of surface immobilized DNA orientation
Author :
Spuhler, Philipp ; Sola, Laura ; Zhang, Xirui ; Monroe, Margo ; Greenspun, Joseph ; Chiari, Marcella ; Unlu, M. Selim
Author_Institution :
Biomed. Eng., Boston Univ., Boston, MA, USA
Abstract :
We utilize spectral self-interference fluorescent microscopy (SSFM) to measure fluorophore height with sub-nm precision to precisely quantify DNA orientation and conformation. A novel polymeric 3D scaffold is used to functionalize the sensor surface and permits controlled orientation of the surface anchored DNA.
Keywords :
DNA; biocontrol; biosensors; fluorescence; molecular biophysics; molecular configurations; optical microscopy; polymers; DNA conformation; SSFM; fluorophore height; polymeric 3D scaffold; precise quantification; sensor; spectral self-interference fluorescent microscopy; surface immobilized DNA orientation surface immobilized DNA orientation; Bioinformatics; DNA; Fluorescence; Polymers; Probes; Proteins; Surface charging;
Conference_Titel :
General Assembly and Scientific Symposium, 2011 XXXth URSI
Conference_Location :
Istanbul
Print_ISBN :
978-1-4244-5117-3
DOI :
10.1109/URSIGASS.2011.6051351
