A study of material incorporation for medaka (Oryzias latipes) EGGS by various voltage pulses

The purpose of this study is to investigate the effects of pulsed electric fields on fertilized eggs and to establish a technique of incorporating materials into them without damage to inside tissues, embryo-cell and yolk. Medaka (Oryzias latipes) egg was used, since its growth can be observed easily with a microscope because of its transparency of outer-shell. In addition, it is easy to handle and breed them. Then a chemical substance called cycloheximide, which is a protein synthesis inhibitor, was selected as the first material to be incorporated. If it is succeeded to incorporate into egg, embryogenesis will be obstructed or delayed directly.