Optical protein chip as microarrays for protein interaction determination

One kind of optical protein chip and a ProteinChip analysis system have been introduced. The basic principle is that one kind of optical imaging technique - imaging ellipsometry is used to visualize optical biochips with the lateral thickness (or surface concentration) distribution of protein layers attached on a patterned surface. The chip is processed with surface patterning, surface modification and ligand immobilization to form a sensing surface with multi-bioactivity. The affinity between proteins is used to realize non-labeling micro-assays for protein identifications and protein interactions. A model of the chip and some demonstration results are presented.