Design and fabrication of microfluidic chip with micro/nano structures

In this manuscript we designed and fabricated two types of microfluidic chips for plasma isolation from whole blood by using micro/nano structures. One type of the microchips was designed based on the principle of crossflow filtration, while the other was based on plasma skimming effect. The microchips were fabricated by MEMS technology, which were made of a silicon wafer and a PDMS-glass cover. The fabrication parameters were demonstrated and optimized for the both types of chips. These microfluidic chips used for plasma isolation has the potential to integrate into micro total analytical system (muTAS) with the advantages of low cost, short analytical time, disposability, low reagent and sample consumption, which will create a microanalysis system for point-of-care diagnostics.