DNA ligation using coplanar electrodes electro-wetting-ondielectric (EWOD) device

This paper reports the completion of fundamental fluidic operation considered essential to build digital microfluidics system, which can be used for micro total analysis system (mu-TAS) or lab-on-a-chip by the coplanar electrode type of EWOD device. Coplanar electrode type of EWOD chip could be designed as a novel tool to delivery many kinds of bio-samples, such as DNA, enzyme, and salt solution without problems. In this study, we present a successful bio-molecular ligation procedure by using coplanar electrodes EWOD chip. The results was shown that the digital microfluidics system based on coplanar electrodes EWOD device can be good enough in the ligation process and the efficiency of ligation was comparable with the one by the conventional practice of pipette. In addition, we also conferred with the efficiency of DNA ligation by varied mixing sequences and waiting time.