Battery-powered miniaturized electroporation system for in-situ gene transfer in stem cell research

Author

He, Huiqi ; Chang, Donald C. ; Lee, Yi-Kuen

Author_Institution

Bioengineering Graduate Program, The Hong Kong University of Science and Technology, Hong Kong

fYear

2009

fDate

5-8 Jan. 2009

Firstpage

463

Lastpage

467

Abstract

A battery-powered miniaturized electroporation (EP) system was designed and fabricated to tackle the challenging DNA transfection of stem cells. The system consists of a programmable mini-electroporator and a micro EP chip. Large statistical data of loading efficiency for five different size molecules and cell viability were determined under various voltage and duration in this system for HeLa cell and MEF cell line. Based on more than 2,000 single-cell electroporation experiments, an EP phase diagram for MEF cells have been generated for the first time. It can be used to significantly increase the in-situ gene transfer efficiency. In addition, the EP boundary curve for MEF cells can be described by a simple empirical formula. Comparing to the conventional instrument, the miniaturized electroporation system can greatly shorten the experimental time. We also proposed a new system for continuous online monitoring in-situ gene expression, which is still in progress,

Keywords

Cells (biology); DNA; Embryo; Fabrication; Gold; Instruments; Monitoring; Pulsed power supplies; Safety; Stem cells; Electroporation; MEF cells; in-situ gene transfer; phase diagram; stem cell;

fLanguage

English

Publisher

ieee

Conference_Titel

Nano/Micro Engineered and Molecular Systems, 2009. NEMS 2009. 4th IEEE International Conference on

Conference_Location

Shenzhen, China

Print_ISBN

978-1-4244-4629-2

Electronic_ISBN

978-1-4244-4630-8

Type

conf

DOI

10.1109/NEMS.2009.5068619

Filename

5068619