Title :
A Portable Two-photon Fluorescence Microendoscope Based on a Two-dimensional Scanning Mirror
Author :
Piyawattanametha, Wibool ; Cocker, Eric D. ; Barretto, Robert P J ; Jung, Juergen C. ; Flusberg, Benjamin A. ; Ra, Hyejun ; Solgaard, Olav ; Schnitzer, Mark J.
Author_Institution :
Stanford Univ., Stanford
fDate :
Aug. 12 2007-July 16 2007
Abstract :
Towards overcoming the size limitations of conventional two-photon fluorescence microscopy for brain imaging in freely moving mice, we introduce a portable laser-scanning microendoscope based on a microelectromechanical systems (MEMS) two-dimensional (2-D) scanning mirror, compound gradient refractive index (GRIN) micro-lenses, and a photonic bandgap fiber (PBF). The microendoscope achieves fast line scanning acquisition rates up to 3.5 kHz and micron-scale imaging resolution.
Keywords :
biomedical optical imaging; brain; endoscopes; fibre optic sensors; gradient index optics; image resolution; laser applications in medicine; micro-optomechanical devices; microlenses; micromirrors; neurophysiology; optical microscopy; photonic band gap; two-photon processes; brain imaging; compound gradient refractive index; frequency 3.5 kHz; laser-scanning microendoscope; mice; microelectromechanical systems; microlenses; micron-scale imaging resolution; photonic bandgap fiber; portable two-photon fluorescence microendoscope; scanning acquisition rates; two-dimensional scanning mirror; two-photon fluorescence microscopy; Brain; Fiber lasers; Fluorescence; Mice; Microelectromechanical systems; Micromechanical devices; Microscopy; Mirrors; Refractive index; Two dimensional displays; 3-D image; fluorescence image; scanner; scanning mirror; two-photon;
Conference_Titel :
Optical MEMS and Nanophotonics, 2007 IEEE/LEOS International Conference on
Conference_Location :
Hualien
Print_ISBN :
978-1-4244-0641-8
DOI :
10.1109/OMEMS.2007.4373812
