Optical transfer function-based micro image enhancement algorithm

The edge and detail information´s losing, image distortion and Ringingeffect pose many challenges to existing micro image enhancement algorithms. In this paper, a novel optical transfer function-based micro image enhancement algorithm is put forward. In this algorithm, the point spread function was acquired according the incoherent illuminate in the optical system firstly. Secondly, the optical transfer function(OTF) was obtained and the high-pass filter based on optical property was constructed through the microscopic OTF. Finally, micro image would be processed by using the compensating filter. As a result, the clear and non-obvious Ringingeffect micro image was gained. Further more, the micro image enhancement algorithm based on OTF was compared with image enhancement algorithm based on Butterworth high-pass filter. Experimental results show that the optical transfer function-based micro image enhancement algorithm can produce a better micro image enhancement effect.