Biosensor impedimétrico para la detección de lindano

In general, organochlorine pesticides detection involves complex and expensive procedures. The aim of this paper is to present an optimized design of a bacterial biosensor to quantify lindane at low concentrations (μg L^-1) based on a Streptomyces sp. M7 spores platform employing impedance spectroscopy as the transduction method. Using this technique, the bacterial metabolic activity was evaluated by analyzing a set of electrical parameters calculated from the impedance records using an equivalent electrical circuit. The results indicate that the changes in the charge transfer resistance are directly related to the bacterial metabolic activity; i.e., lindane consumption. The bacterial impedimetric biosensor design is simple, inexpensive and can detect changes in the lindane concentration as low as 120 μg L^-1 in less than 2 days without previous sample treatment.