Fractal Dimension of Mie Scattering Spectra for the Appraisal of Infected HeLa Cells in Cultures

With the aim of evaluating viral infection effects on cellular structures three fractal methods (Petrosian, Hurst exponent and Height-height correlation) were performed as an expression of quantitative measure of cell nuclei size distribution. Cell nuclei size distribution was determined measuring Mie scattering spectra registered using an original LSS system. Fractal dimension for interest area of Mie scattering spectra was computed using a software package, which can store and process recorded data. The results indicate obviously higher values of fractal dimension for HSV virus infected biological samples compared to non-infected biological samples. This allows us clearly discriminate between virus infected and non-infected biological samples.