Title :
4D spatial-spectral super-resolution imaging of nanoscopic membrane signalling domains
Author :
Baddeley, David ; Jayasinghe, Isuru ; Kong, Cherrie ; Crossman, David ; Cheyne, Juliette ; Montgomery, Johanna ; Cannell, Mark ; Soeller, Christian
Author_Institution :
Dept. of Physiol., Univ. of Auckland, Auckland, New Zealand
fDate :
Aug. 28 2011-Sept. 1 2011
Abstract :
We present a practical implementation of single-molecule based localisation microscopy by combining single laser line excitation and use of conventional dyes. We investigate the organization of membrane proteins into clusters in cardiac and neuronal cells.
Keywords :
biomedical optical imaging; cardiology; image resolution; optical microscopy; 4D spatial-spectral superresolution imaging; cardiac cells; conventional dyes; nanoscopic membrane signalling domain; neuronal cells; single laser line excitation; single molecule based localisation microscopy; Calcium; Microscopy; Proteins; Signal resolution; Spatial resolution;
Conference_Titel :
Quantum Electronics Conference & Lasers and Electro-Optics (CLEO/IQEC/PACIFIC RIM), 2011
Conference_Location :
Sydney, NSW
Print_ISBN :
978-1-4577-1939-4
DOI :
10.1109/IQEC-CLEO.2011.6194007
