Strength of attachment of an in-house, microbubble, ultrasound contrast agent

Author

Edgeworth, A.L. ; Anderson, T. ; Ross, J.A. ; Ansell, I.F. ; Butler, M. ; Norrie, L. ; McDicken, W.N. ; Moran, C.M.

Author_Institution

Med. Phys. & Med. Eng., Univ. of Edinburgh, Edinburgh, UK

fYear

2009

fDate

20-23 Sept. 2009

Firstpage

1262

Lastpage

1265

Abstract

An in-house, lipid-based, microbubble ultrasound contrast agent has been successfully targeted to a streptavidin coated agar surface and SK-Hep-1 cells. Initial investigations assessed the strength of attachment of the agent to an agar surface via a streptavidin-biotin bond. The strength of this bond was found to be 75 times stronger than a control investigation in which the biotinylated agent was `attached´ to an uncoated agar surface. The in-house agent was then targeted to SK-Hep-1 cells using a streptavidin-biotin bridge. Streptavidin was found to attach to over 90% of the microbubble population with antibodies successfully attaching to 75.4% of the microbubble population. Adhesion of SK-Hep-1 cells under low wall shear stress has also been assessed.

Keywords

adhesion; biochemistry; biomedical ultrasonics; bubbles; cellular biophysics; molecular biophysics; ultrasonic imaging; SK-Hep-1 cells; adhesion; antibodies; attachment strength; biotinylated agent; in-house microbubble contrast agent; low wall shear stress; microbubble population; streptavidin coated agar surface; streptavidin-biotin bond; ultrasound contrast agent; Arteries; Backscatter; Biomedical imaging; Bonding; Bridges; Microscopy; Myocardium; Stress; Strips; Ultrasonic imaging; attachment; contrast; high-frequency ultrasound; microbubble; targeting;

fLanguage

English

Publisher

ieee

Conference_Titel

Ultrasonics Symposium (IUS), 2009 IEEE International

Conference_Location

Rome

ISSN

1948-5719

Print_ISBN

978-1-4244-4389-5

Electronic_ISBN

1948-5719

Type

conf

DOI

10.1109/ULTSYM.2009.5441442

Filename

5441442