Title :
A comparison of deglycerolization processes
Author :
Reddy, H. ; Draheim, J. ; Rudolph, D. ; Coughlin, R.
Author_Institution :
SymBiotech. Inc., Wallingford, CT, USA
Abstract :
This study compared a standard centrifugation based method for deglycerolizing cryopreserved RBCs and a proprietary, automated method based upon hollow fiber membrane (HFM) filtration. The methods were compared by examining the characteristics (i.e. glycerol concentration, quantity of RBC and hemoglobin, osmotic fragility) of the product of each. Deglycerolization with the filtration method yielded products with final glycerol concentrations less than 1% w/v, as did the centrifugation. Deglycerolized RBC units from each process exhibited no significant difference in the osmotic fragility or in the quantity of RBCs and hemoglobin available (99% level)
Keywords :
biological techniques; blood; cellular biophysics; filtration; freezing; osmosis; automated method; cell freezing; cryopreserved red blood cells; deglycerolization processes; final glycerol concentration; glycerol concentration; hemoglobin quantity; hollow fiber membrane filtration; long-term storage; osmotic fragility; standard centrifugation based method; sterility; Assembly; Cells (biology); Extracellular; Filtration; Gravity; Ice; Laboratories; Protocols; Red blood cells; Testing;
Conference_Titel :
Bioengineering Conference, 2000. Proceedings of the IEEE 26th Annual Northeast
Conference_Location :
Storrs, CT
Print_ISBN :
0-7803-6341-8
DOI :
10.1109/NEBC.2000.842406
