Contactless fluorescence imaging with a CMOS image sensor

In this work, we utilize a CMOS active pixel sensor in a fluorescence imaging setup. The ability to sense small light intensity changes on top of a large baseline with spatial resolution at the subcellular scale is required in fluorescence imaging. The CMOS imager presented in [1] is perfect for this application with the ability to resolve fine features coupled with high dynamic range. By using a custom imager with a relay lens we are able to realize a dramatic decrease in device size, cost and complexity of the whole system.