Microalgae, M. aeruginosa as a renewable resource for bioalcohol production

M. aeruginosa is useful resource for bioalcohol production without any competition with foodstuff, and it bear fermentable higher carbohydrates(56.4°/o) such as sucrose, cellobiose, rhamnose, galactose, xylose and glucose, and new medium for M. aeruginosa was developed with added urea 0.2g/L, increased Fe⁺², and decreased Ca⁺² ion compared with BG11 medium. After culture with 25L and 240 Utter race-way reactor, M. aeruginosa was precipitated with combination of Al₂SO₄ and FeCl₃, precipitates were hydrolyzed with C-H₂SO₄, followed autoclaved and sonication, finally the extract was used for substrate of alcohol fermentation, 120mg/ml of sugar in BG11 medium, and 140 mg/ml in modified medium were produced. Fermentation was performed with 200 ml extract in 500 ml flask at 30°C for 30 hours, with 3 kinds of strain, Saccharomyces cerevisiae(Sc), Pichia stipitis(Ps), Zymomonas mobilis(Zm), and mixed these strains(Sc+Ps+Zm). Pichia stipitis(0.7%) is more suitable for producing bioalcohol for M. aeruginosa extract than other strains of Saccharomyces cerevisiae(0.45%) and Zymomonas mobilis(0.61%), while co-cultured strains showed highest product by 1.75%. M. aeruginosa will be a renewable resource for bioalcohol fermentation.