Thyroglobulin detection using competitive protein adsorption

Author

Choi, Seokheun ; Chae, Junseok

Author_Institution

Sch. of Electr., Comput. & Energy Eng., Arizona State Univ., Tempe, AZ, USA

fYear

2010

fDate

24-28 Jan. 2010

Firstpage

887

Lastpage

890

Abstract

We report a unique sensing mechanism based on competitive proteins´ adsorption/exchange reaction, namely Vroman effect, for detecting a cancer biomarker, thyroglobulin (Tg). Implemented in a microfluidic system, the target protein, Tg, displaces a pre-adsorbed weak-affinity protein, IgG, on one surface, while a pre-adsorbed strong-affinity protein, fibrinogen, is not displaced by Tg on the other surface. Differential measurement using SPR (Surface Plasmon Resonance) allows the detection of Tg; the SPR angle changes offer a quantitative analysis of concentration of Tg in a sample. Using this technique, we demonstrate the selective detection of Tg in a protein mixture of albumin, haptoglobin, IgG and Tg.

Keywords

adsorption; bioMEMS; biochemistry; biomedical measurement; biosensors; cancer; ion exchange; microfluidics; molecular biophysics; optical sensors; proteins; surface plasmon resonance; IgG; Tg; Vroman effect; adsorption; albumin; cancer biomarker; competitive protein adsorption; differential measurement; exchange reaction; fibrinogen; haptoglobin; microfluidic system; microfluidic systems; pre-adsorbed strong-affinity protein; pre-adsorbed weak-affinity protein; surface plasmon resonance; target protein; thyroglobulin detection; Biomarkers; Biosensors; Cancer detection; Glass; Gold; Microfluidics; Optical films; Plasmons; Protein engineering; Resonance;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2010 IEEE 23rd International Conference on

Conference_Location

Wanchai, Hong Kong

ISSN

1084-6999

Print_ISBN

978-1-4244-5761-8

Electronic_ISBN

1084-6999

Type

conf

DOI

10.1109/MEMSYS.2010.5442349

Filename

5442349