A high performance electroporation chip integrating multi-well plate and annular interdigital microelectrodes

Electroporation, in which electric pulses are applied to create transient pores in the cell membrane, is widely used to introduce biologically active molecules into cells. Here we report a novel electroporation chip with great performance and good compatibility with the standard multi-well plate used in biological research. We demonstrated a prototype chip integrating with a 12-well plate and successfully electroporated both adherent and supernatant cells. Using the standard expression cell line HEK-293 and expression vector pEGFP-C3, we realized excellent cell viability (80%) and transfection rate (90%), both much higher than those in previously reported methods.