Title :
A DNA-extraction and polymerase-chain-reaction microchip using magnetic beads and thermo-pneumatic valves
Author :
Chia, Bonnie T. ; Yang, Xing-Ying ; Cheng, Ming-Yuan ; Yang, Yao-Joe
Author_Institution :
Nat. Taiwan Univ., Taipei, Taiwan
Abstract :
In this work, we develop a miniaturized system for DNA extraction and polymerase chain reaction (PCR). DNA is extracted from whole blood sample by using silica coated magnetic beads. Electromagnetic actuation is employed to manipulate the magnetic beads for transporting DNA molecules in fluidic channels. Thermo-pneumatic valves with low temperature elevation are integrated into the microfluidic chip for isolating/releasing fluidic samples and reagents. The size of the system is 72mm×54mm×11mm. The device fully operates with a 12V DC voltage and automatically performs DNA extraction and amplification processes after samples and reagents are loaded.
Keywords :
DNA; microfluidics; valves; DNA-extraction; amplification processes; electromagnetic actuation; fluidic channels; low temperature elevation; magnetic beads; polymerase-chain-reaction microchip; silica coated magnetic beads; thermopneumatic valves; Coils; DNA; Distributed control; Heating; Magnetic analysis; Microfluidics; Polymers; Purification; Temperature; Valves;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2010 IEEE 23rd International Conference on
Conference_Location :
Wanchai, Hong Kong
Print_ISBN :
978-1-4244-5761-8
Electronic_ISBN :
1084-6999
DOI :
10.1109/MEMSYS.2010.5442369
