Micro-CEC chip with gradient hydrophobic stationary phase (GHSP) provided by mwcnts nanocolumns-applied to protein analysis in MALDI-TOF-MS

Author

Lin, Hsuan-Hsuan ; Wu, Ren-Guei ; Chen, Hsiao-Yu ; Yang, Chung-Shi ; Tseng, Fang-Gang

Author_Institution

Eng. & Syst. Sci. Dept., Nat. Tsing Hua Univ., Hsinchu, Taiwan

fYear

2010

fDate

24-28 Jan. 2010

Firstpage

975

Lastpage

978

Abstract

In this research, we present a chip based capillary electrochromatography (CEC) carrying out a highly efficient separation of biomolecules through gradient hydrophobic stationary phase (GHSP) provided by multiwalled carbon nanotubes (MWCNTs) nanocolumns. 4-azidoaniline was employed for the surface modification of the MWCNTs to form a gradient stationary phase by a photochemical reaction through a gradient light filter. This device was used for separating proteins (20 Â¿M FITC-BSA and FITC-Cytochrome C) with different hydrophobic characteristics, and MALDI-TOF-MS detection was employed to verify the separation efficiency in this MWCNTs nanocolumn. The best separation resolution of the FITC-BSA column efficiency was 2.5 Ã— 10⁴ (plats/m).

Keywords

bioMEMS; biochemistry; carbon nanotubes; chromatography; electrophoresis; hydrophobicity; lab-on-a-chip; macromolecules; molecular biophysics; optical filters; osmosis; photochemistry; proteins; surface treatment; 4-azidoaniline; MWCNT nanocolumns; biomolecules; capillary electrochromatography; electrokinetic separations; electroosmotic pumping; gradient hydrophobic stationary phase; gradient light filter; micro-CEC chip; microfluidic channels; multiwalled carbon nanotube nanocolumns; photochemical reaction; protein analysis; surface modification; Carbon nanotubes; Electrochromism; Electrokinetics; Molecular biophysics; Nanobioscience; Phased arrays; Protein engineering; Proteomics; Reservoirs; Systems engineering and theory;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2010 IEEE 23rd International Conference on

Conference_Location

Wanchai, Hong Kong

ISSN

1084-6999

Print_ISBN

978-1-4244-5761-8

Electronic_ISBN

1084-6999

Type

conf

DOI

10.1109/MEMSYS.2010.5442371

Filename

5442371