Title :
Lab-in-a-trench platform for real-time monitoring of cell surface protein expression
Author :
Dimov, Ivan K. ; Kijanka, Gregor ; Ducrée, Jens
Author_Institution :
Nat. Centre for Sensor Res., Dublin City Univ., Dublin, Ireland
Abstract :
In this work we for the first time demonstrate real-time monitoring of the expression of membrane proteins in native, live cells, free of hydrodynamic stress at single cell resolution. This micro-optofluidic mechanism is uniquely enabled by the intricate interplay of gravity induced sedimentation with laminar flow, fast diffusion and short optical path length on our lab-in-a-trench platform.
Keywords :
bioMEMS; biomembranes; cellular biophysics; lab-on-a-chip; microfluidics; proteins; cell surface protein expression; diffusion; gravity induced sedimentation; hydrodynamic stress; lab-in-a-trench platform; laminar flow; membrane proteins; microoptofluidic mechanism; optical path length; real time monitoring; Biomedical engineering; Biomedical monitoring; Biosensors; Cells (biology); Electrokinetics; Gravity; Hydrodynamics; Microfluidics; Protein engineering; Stress;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2010 IEEE 23rd International Conference on
Conference_Location :
Wanchai, Hong Kong
Print_ISBN :
978-1-4244-5761-8
Electronic_ISBN :
1084-6999
DOI :
10.1109/MEMSYS.2010.5442556
