Lab-in-a-trench platform for real-time monitoring of cell surface protein expression

Author

Dimov, Ivan K. ; Kijanka, Gregor ; Ducrée, Jens

Author_Institution

Nat. Centre for Sensor Res., Dublin City Univ., Dublin, Ireland

fYear

2010

fDate

24-28 Jan. 2010

Firstpage

96

Lastpage

99

Abstract

In this work we for the first time demonstrate real-time monitoring of the expression of membrane proteins in native, live cells, free of hydrodynamic stress at single cell resolution. This micro-optofluidic mechanism is uniquely enabled by the intricate interplay of gravity induced sedimentation with laminar flow, fast diffusion and short optical path length on our lab-in-a-trench platform.

Keywords

bioMEMS; biomembranes; cellular biophysics; lab-on-a-chip; microfluidics; proteins; cell surface protein expression; diffusion; gravity induced sedimentation; hydrodynamic stress; lab-in-a-trench platform; laminar flow; membrane proteins; microoptofluidic mechanism; optical path length; real time monitoring; Biomedical engineering; Biomedical monitoring; Biosensors; Cells (biology); Electrokinetics; Gravity; Hydrodynamics; Microfluidics; Protein engineering; Stress;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2010 IEEE 23rd International Conference on

Conference_Location

Wanchai, Hong Kong

ISSN

1084-6999

Print_ISBN

978-1-4244-5761-8

Electronic_ISBN

1084-6999

Type

conf

DOI

10.1109/MEMSYS.2010.5442556

Filename

5442556