Title :
A free-standing and flexible parylene PCR device
Author :
Satsanarukkit, Penvipha ; Lo, Hsiwen ; Tai, Yu Chong
Author_Institution :
Dept. of Electr. Eng., California Inst. of Technol., Pasadena, CA, USA
Abstract :
We present a free-standing and flexible parylene-channel PCR device that allows a simple sealing mechanism to prevent bubbling caused by PCR temperature cycling. This sealing mechanism saves complicated, time-consuming in-channel valve fabrication processes and achieves excellent sealing performance. It is proven experimentally that this new PCR device plus its unique packaging is capable of locking PCR solution in the PCR chamber at 95°C for at least 5 hours without bubbling and leaking. As a result, we have successfully obtained the on chip PCR amplification without the bubble and leaking problem.
Keywords :
microfluidics; microsensors; polymers; PCR temperature cycling; bubbling; flexible parylene PCR device; free-standing parylene PCR device; in-channel valve fabrication process; leaking problem; on chip PCR amplification; polymerase chain reaction; sealing mechanism; temperature 95 C; Fluorescence; Heating; Lasers; Microchannel; Nanoelectromechanical systems; Phase change random access memory; Silicon; PCR; bubbling and leaking problems; microfluidics; parylene-C;
Conference_Titel :
Nano/Micro Engineered and Molecular Systems (NEMS), 2012 7th IEEE International Conference on
Conference_Location :
Kyoto
Print_ISBN :
978-1-4673-1122-9
DOI :
10.1109/NEMS.2012.6196743
