The effect of Cytochalasin D on F-Actin behavior of single-cell electroendocytosis using multi-chamber micro cell chip

Author

Lin, Ran ; Chang, Donald C. ; Lee, Yi-Kuen

fYear

2012

fDate

5-8 March 2012

Firstpage

150

Lastpage

153

Abstract

Electroendocytosis (EED) is a pulsed-electric-field (PEF) induced endocytosis, facilitating cells uptake molecules through nanometer-sized EED vesicles. We herein investigate the effect of a chemical inhibitor, Cytochalasin D (CD) on the actin-filaments (F-Actin) behavior of single-cell EED. The CD concentration (C_CD) can control the depolymerization of F-actin. A multi-chamber micro cell chip was fabricated to study the EED under different conditions. Large-scale single-cell data demonstrated EED highly depends on both electric field and C_CD.

Keywords

adsorption; bioMEMS; biochemistry; bioelectric phenomena; cellular biophysics; lab-on-a-chip; molecular biophysics; polymerisation; proteins; F-actin behavior; actin filaments; biomolecules; chemical inhibitor; cytochalasin D; depolymerization; multichamber microcell chip; pulsed-electric-field induced endocytosis; single-cell electroendocytosis; Fluorescence; Gold; Nanoelectromechanical systems; Polymers; Actin filament; Cytochalasin D; Electroendocytosis; Micro Cell Chip;

fLanguage

English

Publisher

ieee

Conference_Titel

Nano/Micro Engineered and Molecular Systems (NEMS), 2012 7th IEEE International Conference on

Conference_Location

Kyoto

Print_ISBN

978-1-4673-1122-9

Type

conf

DOI

10.1109/NEMS.2012.6196744

Filename

6196744