Cryogenic frozen device for hepatocyte culture and responses

In clinical medicine, freezing treatment has been applied to patients for years. However, the combination of the micro/nano biochip (or Lab Chip) techniques and cryogenic frozen techniques were seldom proposed in the past. This paper reports a cryogenic frozen apparatus for the study of microfluidic liver tissue mimic hepatic cords responses on three dimension hepatocyte culture chip. We designed the micro-cylinder about the height of 85 micrometer for loading hepatocyte to form three dimension tissue-mimic structures. The liver is organized into lobules which take the shape of polygonal prisms. Each lobule is typically hexagonal in cross section and is centered on the central vein. The bulk of the lobule consists of hepatocytes which are arranged into hepatic cords that are separated by sinusoid space. In liver function testing, the albumin secretion was affected 12% by freezing the hepatocyte ten minutes.