L methodology for high precision fabrication

In this paper a neural interface has been designed, in terms of materials, layout and surface morphology. A methodology for its fabrication and characterization has been provided. This methodology is based on the use of focused ion beam (FIB), atomic force microscope (AFM) and micromanipulators, respectively for nano-modification, topographical characterization and nano-dispensing of liquid biological sample. The proposed design and methodology satisfy all the constraints, specifications and requirements coming from neurophysiology issues. Analytical techniques and in vitro testing (with PC 12 cell line) provided the criteria for the validation of the proposed solutions. Preliminary in vitro testing allows to conclude that the developed neural interface satisfies the elementary issues of biocompatibility. Further studies will be performed via AFM to investigate how biological matter interacts with nano-modified electrodes. The influence of topographical modifications of the electrode will be studied on neural cells as well as on proteins or other macromolecules which mediate the cell adhesion on an implantable surface