3D Fluorescent Spots Detection in Line-Scanning Confocal Microscopy

Author

Dusch, E. ; Vincent, N. ; Genovesio, A.

Author_Institution

Univ. Rene Descartes Paris 5, Paris

Volume

6

fYear

2007

fDate

Sept. 16 2007-Oct. 19 2007

Abstract

This paper describes a method to detect fluorescent PSF-like spots in 3D line-scanning confocal microscopy. We suppose that fluorescent spots are small enough to be characterized by the Point Spread function (PSF) of the microscope. To localize spots, we use a derivative expression of the image previously convolved by a Gaussian approximating the PSF. The algorithm validation is made on 3D synthetic images with fluorescent background structures and on a 3D fluorescent biological image.

Keywords

Gaussian processes; approximation theory; biomedical optical imaging; medical image processing; object detection; optical microscopy; 3D fluorescent biological image; 3D fluorescent spot detection; 3D line-scanning confocal microscopy; Gaussian approximation; point spread function; Background noise; Biological system modeling; Biomedical optical imaging; Filtering; Fluorescence; Gaussian approximation; Lighting; Object detection; Optical microscopy; Photobleaching; 3D Point-Spread-Function; Biomedical microscopy; Hessian operator; Image object detection; Line-Scanning Confocal Microscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Image Processing, 2007. ICIP 2007. IEEE International Conference on

Conference_Location

San Antonio, TX

ISSN

1522-4880

Print_ISBN

978-1-4244-1437-6

Electronic_ISBN

1522-4880

Type

conf

DOI

10.1109/ICIP.2007.4379566

Filename

4379566