Title :
The interaction between functionalized ZnS nanofluorescence probe and DNA
Author :
Tian-Wei, Jiang ; Yue-Qing, Gu ; Ping, Wang
Author_Institution :
Biosensor Nat. Special Lab., Zhejiang Univ., Hangzhou
Abstract :
A novel method was developed for the determination of DNA. The method was based on the mechanism of interaction between functionalized nanoparticle ZnS and deoxyribonucleic acid (DNA). Fluorescence intensity of ZnS is quenched by DNA at medium pH. The optimal condition was chosen for DNA and fluorescence spectrometry was used. Linear relationship between the quenched fluorescence intensity and the concentration of DNA was found in the range of 0.2-1.2 ug/L. The limit of detection was 0.17 ug/L. The method is simple and shows high sensitivity. Interaction between DNA and thioglycolic acid-ZnS existed.
Keywords :
DNA; II-VI semiconductors; bio-optics; biological techniques; fluorescence; molecular biophysics; nanobiotechnology; nanoparticles; radiation quenching; zinc compounds; DNA; ZnS; deoxyribonucleic acid; fluorescence spectrometry; functionalized nanoparticle; nanofluorescence probe; quenched fluorescence intensity; thioglycolic acid; Biosensors; DNA; Fluorescence; Instruments; Laboratories; Nanoparticles; Pharmaceuticals; Probes; Spectroscopy; Zinc compounds; DNA; fluorescence probe; nanoparticle; thioglycolic acid;
Conference_Titel :
Biophotonics, Nanophotonics and Metamaterials, 2006. Metamaterials 2006. International Symposium on
Conference_Location :
Hangzhou
Print_ISBN :
0-7803-9773-8
Electronic_ISBN :
0-7803-9774-6
DOI :
10.1109/METAMAT.2006.334918
