Design of a PMMA Chip for Selective Extraction of Size-Fractioned DNA

Fraction collection following DNA electrophoresis has many applications in biology analysis. These assays typically need to identify specific DNA fragments in the separated sample for further processing and require extraction of one or a group of fragments. Unfortunately, the conventional methods involve cumbersome procedure and are not amenable to integration, automation and extraction of microscale samples. In this work, we present a scheme of chip-based electroelution for the extraction of DNA fragments, which allows the isolation and collection of target DNA fragments in a single device. In order to improve the purity and yield of the extracted DNA fragment, we proposed an optimal channel configuration for DNA recovery chip. Both theoretical analysis and experimental results revealed that the efficiency of DNA extraction was achieved with the optimized chip design