Disposable Biosensor for Hemoglobin Determination in Whole Blood

Author

Jiang, Liying ; Luo, Xianbo ; Tian, Qing ; Wang, Hui ; Cai, Xinxia

Author_Institution

State Key Lab of Transducer Technol., Chinese Acad. of Sci., Beijing

fYear

2006

fDate

18-21 Jan. 2006

Firstpage

204

Lastpage

208

Abstract

A disposable biosensor for the determination of hemoglobin in whole blood is described. The sensing method is based on amperometric detection technology. Platinized thin-film gold electrode is employed as a base electrode for the immobilization of mediator potassium ferricyanide. Saponin hemolyses the erythrocytes and hemoglobin is released. The biosensor has characters of rapid response time (60 s), high accuracy (> 90%), small sample volume (3 muL) and good precision (CV = 3.6%). The biosensor connected with the homemade portable meter can be used to determine hemoglobin concentration in clinical diagnosis and home-care purpose for athletes and patients potentially

Keywords

amperometric sensors; biosensors; blood; electrochemical analysis; gold; platinum; potassium; proteins; 60 s; Au; Pt; amperometric detection technology; athletes; clinical diagnosis; disposable biosensor; erythrocytes; hemoglobin concentration; hemoglobin determination; homecare application; homemade portable meter; patients; platinized thin film gold electrode; potassium ferricyanide; saponin hemolysis; whole blood; Biosensors; Blood; Chemical analysis; Chemical technology; Diseases; Electrodes; Gold; Instruments; Platinum; Sputtering; disposable biosensor; hemoglobin; portable meter;

fLanguage

English

Publisher

ieee

Conference_Titel

Nano/Micro Engineered and Molecular Systems, 2006. NEMS '06. 1st IEEE International Conference on

Conference_Location

Zhuhai

Print_ISBN

1-4244-0139-9

Electronic_ISBN

1-4244-0140-2

Type

conf

DOI

10.1109/NEMS.2006.334687

Filename

4134935